Lincoln will be without midfielder Billy Knott after their appeal against his red card at Notts County was rejected by the Football Association.
Manager Danny Cowley had hoped to have Knott available to face Barnet but he will miss three games.
He was sent off in the 28th minute of Saturday's 4-1 defeat at Meadow Lane after appearing to catch County's Ryan Yates in the face with a high boot.
Knott's absence will force Cowley into a change in his starting line-up against the Bees and he could bring in either Ollie Palmer or Matt Rhead to partner Matt Green up front.
Barnet are still without striker John Akinde as last season's top scorer battles a hamstring injury.
However, Rossi Eames should be boosted by the return of Ricardo Santos, who missed Saturday's home defeat to Crawley with a dead leg.
Jamie Stephens and Curtis Weston (both knee) are close to a return, although Tuesday' clash is likely to come too soon.
Forward Dave Tarpey, Elliott Johnson (both knee) and Richard Brindley (foot) are all long-term absentees.